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Article Title: TSC2-mTORC1 axis regulates morphogenesis and neurological function of Gli1<sup>+</sup> adult-born dentate granule cells
Article Snippet: Aberrant adult hippocampal neurogenesis is implicated in neurological and mood disorders associated with dysregulation of the mechanistic target of rapamycin (mTOR).. Understanding how the mTOR pathway shapes the functional development of different subpopulations of adultborn hippocampal neural stem cells will enable insight into potential therapeutic pathways for these disorders.. Here we study how loss of TSC2, a regulator of mTOR pathway and a causal gene for Tuberous Sclerosis Complex (TSC), affects dentate gyrus granule cell (dGC) morphogenesis and hippocampal-dependent function.
Western Blot:Article Title: The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses.
Article Snippet: .. Primary antibodies used in this studywereobtained from the following sources and used for Western blotting at 1:1000 dilution unless otherwise stated: Rabbit anti-Raptor, Cell Signaling Technology, Cat# 2280 Rabbit anti-cGAS, Cell Signaling Technology, Cat# 15102 Rabbit anti-cGAS, PROTEINTECH, Cat# 26416-1-AP Rabbit anti-STING, Cell Signaling Technology, Cat# 13647 S Rabbit anti-phospho-STING (S366), Cell Signaling Technology, Cat# 85735 S Rabbit anti-MxA, Cell Signaling Technology, Cat# 37849 Rabbit anti-MxB, PROTEINTECH, Cat# 13278-1-AP Rabbit anti-MxB, NOVUS BIOLOGICALS, Cat# NBP1-81018 Rabbit anti-ISG56, Cell Signaling Technology, Cat# 14769 Rabbit anti-ISG54, PROTEINTECH, Cat# 12604-1-AP Sheep anti-TGN46, Bio-Rad, Cat# AHP500 Mouse anti-Flag M2, MilliporeSigma, Cat# F1804 Rabbit anti-Lamin A/C, PROTEINTECH, Cat# 10298-1-AP Rabbit anti-FIS1, PROTEINTECH, Cat# 10956-1-AP Rabbit anti-ATP5a1, PROTEINTECH, Cat# 14676-1-AP Mouse anti-DNA, MilliporeSigma, Cat# CBL186 Rabbit anti-Pink1, Abcam, Cat# ab216144 Rabbit anti-PARK2/Parkin, PROTEINTECH, Cat# 14060-1-AP Rabbit anti-Mitofusin-1, Cell Signaling Technology, Cat# 14739 Rabbit anti-Mitofusin-2, Cell Signaling Technology, Cat# 9482 Rabbit anti-phospho-Mitofusin-2 (S442), Millipore Sigma, Sigma Aldrich, Cat# ABC963 Rabbit anti-VDAC1/2, PROTEINTECH, Cat# 10866-1-AP Rabbit anti-TFAM, PROTEINTECH, Cat# 22586-1-AP Rabbit anti-CytB, PROTEINTECH, Cat# 55090-1-AP Rabbit anti-DRP1, PROTEINTECH, Cat# 12957-1-AP Fig. 6 | Mitochondrial fusion regulates mtDNA release and responses to iF17 infection. a–d NHDFs were treated with control or independent MFN1 siRNAs prior to infection. a RepresentativeWestern blot showingMFN1 depletion reduces ISG responses to iF17 infection but does not affectmtCytB levels.MolecularWeight markers are shown in kDa. b RT-qPCR analysis of fractionated samples showing relative levels of cytosolic mtDNA presented as fold change over control siRNAtreatedmock infected cells, arbitrarily set to 1. n = 3 per group, unpaired two-tailed t-test. ..
Infection:Article Title: The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses.
Article Snippet: .. Primary antibodies used in this studywereobtained from the following sources and used for Western blotting at 1:1000 dilution unless otherwise stated: Rabbit anti-Raptor, Cell Signaling Technology, Cat# 2280 Rabbit anti-cGAS, Cell Signaling Technology, Cat# 15102 Rabbit anti-cGAS, PROTEINTECH, Cat# 26416-1-AP Rabbit anti-STING, Cell Signaling Technology, Cat# 13647 S Rabbit anti-phospho-STING (S366), Cell Signaling Technology, Cat# 85735 S Rabbit anti-MxA, Cell Signaling Technology, Cat# 37849 Rabbit anti-MxB, PROTEINTECH, Cat# 13278-1-AP Rabbit anti-MxB, NOVUS BIOLOGICALS, Cat# NBP1-81018 Rabbit anti-ISG56, Cell Signaling Technology, Cat# 14769 Rabbit anti-ISG54, PROTEINTECH, Cat# 12604-1-AP Sheep anti-TGN46, Bio-Rad, Cat# AHP500 Mouse anti-Flag M2, MilliporeSigma, Cat# F1804 Rabbit anti-Lamin A/C, PROTEINTECH, Cat# 10298-1-AP Rabbit anti-FIS1, PROTEINTECH, Cat# 10956-1-AP Rabbit anti-ATP5a1, PROTEINTECH, Cat# 14676-1-AP Mouse anti-DNA, MilliporeSigma, Cat# CBL186 Rabbit anti-Pink1, Abcam, Cat# ab216144 Rabbit anti-PARK2/Parkin, PROTEINTECH, Cat# 14060-1-AP Rabbit anti-Mitofusin-1, Cell Signaling Technology, Cat# 14739 Rabbit anti-Mitofusin-2, Cell Signaling Technology, Cat# 9482 Rabbit anti-phospho-Mitofusin-2 (S442), Millipore Sigma, Sigma Aldrich, Cat# ABC963 Rabbit anti-VDAC1/2, PROTEINTECH, Cat# 10866-1-AP Rabbit anti-TFAM, PROTEINTECH, Cat# 22586-1-AP Rabbit anti-CytB, PROTEINTECH, Cat# 55090-1-AP Rabbit anti-DRP1, PROTEINTECH, Cat# 12957-1-AP Fig. 6 | Mitochondrial fusion regulates mtDNA release and responses to iF17 infection. a–d NHDFs were treated with control or independent MFN1 siRNAs prior to infection. a RepresentativeWestern blot showingMFN1 depletion reduces ISG responses to iF17 infection but does not affectmtCytB levels.MolecularWeight markers are shown in kDa. b RT-qPCR analysis of fractionated samples showing relative levels of cytosolic mtDNA presented as fold change over control siRNAtreatedmock infected cells, arbitrarily set to 1. n = 3 per group, unpaired two-tailed t-test. ..
Control:Article Title: The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses.
Article Snippet: .. Primary antibodies used in this studywereobtained from the following sources and used for Western blotting at 1:1000 dilution unless otherwise stated: Rabbit anti-Raptor, Cell Signaling Technology, Cat# 2280 Rabbit anti-cGAS, Cell Signaling Technology, Cat# 15102 Rabbit anti-cGAS, PROTEINTECH, Cat# 26416-1-AP Rabbit anti-STING, Cell Signaling Technology, Cat# 13647 S Rabbit anti-phospho-STING (S366), Cell Signaling Technology, Cat# 85735 S Rabbit anti-MxA, Cell Signaling Technology, Cat# 37849 Rabbit anti-MxB, PROTEINTECH, Cat# 13278-1-AP Rabbit anti-MxB, NOVUS BIOLOGICALS, Cat# NBP1-81018 Rabbit anti-ISG56, Cell Signaling Technology, Cat# 14769 Rabbit anti-ISG54, PROTEINTECH, Cat# 12604-1-AP Sheep anti-TGN46, Bio-Rad, Cat# AHP500 Mouse anti-Flag M2, MilliporeSigma, Cat# F1804 Rabbit anti-Lamin A/C, PROTEINTECH, Cat# 10298-1-AP Rabbit anti-FIS1, PROTEINTECH, Cat# 10956-1-AP Rabbit anti-ATP5a1, PROTEINTECH, Cat# 14676-1-AP Mouse anti-DNA, MilliporeSigma, Cat# CBL186 Rabbit anti-Pink1, Abcam, Cat# ab216144 Rabbit anti-PARK2/Parkin, PROTEINTECH, Cat# 14060-1-AP Rabbit anti-Mitofusin-1, Cell Signaling Technology, Cat# 14739 Rabbit anti-Mitofusin-2, Cell Signaling Technology, Cat# 9482 Rabbit anti-phospho-Mitofusin-2 (S442), Millipore Sigma, Sigma Aldrich, Cat# ABC963 Rabbit anti-VDAC1/2, PROTEINTECH, Cat# 10866-1-AP Rabbit anti-TFAM, PROTEINTECH, Cat# 22586-1-AP Rabbit anti-CytB, PROTEINTECH, Cat# 55090-1-AP Rabbit anti-DRP1, PROTEINTECH, Cat# 12957-1-AP Fig. 6 | Mitochondrial fusion regulates mtDNA release and responses to iF17 infection. a–d NHDFs were treated with control or independent MFN1 siRNAs prior to infection. a RepresentativeWestern blot showingMFN1 depletion reduces ISG responses to iF17 infection but does not affectmtCytB levels.MolecularWeight markers are shown in kDa. b RT-qPCR analysis of fractionated samples showing relative levels of cytosolic mtDNA presented as fold change over control siRNAtreatedmock infected cells, arbitrarily set to 1. n = 3 per group, unpaired two-tailed t-test. ..
Quantitative RT-PCR:Article Title: The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses.
Article Snippet: .. Primary antibodies used in this studywereobtained from the following sources and used for Western blotting at 1:1000 dilution unless otherwise stated: Rabbit anti-Raptor, Cell Signaling Technology, Cat# 2280 Rabbit anti-cGAS, Cell Signaling Technology, Cat# 15102 Rabbit anti-cGAS, PROTEINTECH, Cat# 26416-1-AP Rabbit anti-STING, Cell Signaling Technology, Cat# 13647 S Rabbit anti-phospho-STING (S366), Cell Signaling Technology, Cat# 85735 S Rabbit anti-MxA, Cell Signaling Technology, Cat# 37849 Rabbit anti-MxB, PROTEINTECH, Cat# 13278-1-AP Rabbit anti-MxB, NOVUS BIOLOGICALS, Cat# NBP1-81018 Rabbit anti-ISG56, Cell Signaling Technology, Cat# 14769 Rabbit anti-ISG54, PROTEINTECH, Cat# 12604-1-AP Sheep anti-TGN46, Bio-Rad, Cat# AHP500 Mouse anti-Flag M2, MilliporeSigma, Cat# F1804 Rabbit anti-Lamin A/C, PROTEINTECH, Cat# 10298-1-AP Rabbit anti-FIS1, PROTEINTECH, Cat# 10956-1-AP Rabbit anti-ATP5a1, PROTEINTECH, Cat# 14676-1-AP Mouse anti-DNA, MilliporeSigma, Cat# CBL186 Rabbit anti-Pink1, Abcam, Cat# ab216144 Rabbit anti-PARK2/Parkin, PROTEINTECH, Cat# 14060-1-AP Rabbit anti-Mitofusin-1, Cell Signaling Technology, Cat# 14739 Rabbit anti-Mitofusin-2, Cell Signaling Technology, Cat# 9482 Rabbit anti-phospho-Mitofusin-2 (S442), Millipore Sigma, Sigma Aldrich, Cat# ABC963 Rabbit anti-VDAC1/2, PROTEINTECH, Cat# 10866-1-AP Rabbit anti-TFAM, PROTEINTECH, Cat# 22586-1-AP Rabbit anti-CytB, PROTEINTECH, Cat# 55090-1-AP Rabbit anti-DRP1, PROTEINTECH, Cat# 12957-1-AP Fig. 6 | Mitochondrial fusion regulates mtDNA release and responses to iF17 infection. a–d NHDFs were treated with control or independent MFN1 siRNAs prior to infection. a RepresentativeWestern blot showingMFN1 depletion reduces ISG responses to iF17 infection but does not affectmtCytB levels.MolecularWeight markers are shown in kDa. b RT-qPCR analysis of fractionated samples showing relative levels of cytosolic mtDNA presented as fold change over control siRNAtreatedmock infected cells, arbitrarily set to 1. n = 3 per group, unpaired two-tailed t-test. ..
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